Plasmid

Part:BBa_K5302032:Design

Designed by: Dekun Zhou   Group: iGEM24_USTC   (2024-10-01)


pBBR-OmpA-VEGFR1D2-l1masking#18


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2376
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NotI site found at 5308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2376
    Illegal BglII site found at 6054
    Illegal BamHI site found at 1302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2376
    Illegal XbaI site found at 1296
    Illegal PstI site found at 123
    Illegal PstI site found at 1284
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 3034
    Illegal AgeI site found at 1955
    Illegal AgeI site found at 2874
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5630
    Illegal SapI.rc site found at 423
    Illegal SapI.rc site found at 633


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

VEGFR1D2: The second domain of human VEGFR1 OmpA: Escherichia coli (strain K12) VEGFR-masking#18: Rational design

References